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emem  (ATCC)


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    ATCC emem
    Emem, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6383 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eagle+s+minimum+essential+medium+emem/Eagle's+Minimum+Essential+Medium/pm42882443-115-11-14
    Average 99 stars, based on 6383 article reviews
    emem - by Bioz Stars, 2026-11
    99/100 stars

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    Cell Culture:

    Article Title: Methods of generating organoids for high throughput screening of drugs
    Article Snippet: .. U87-MG cells (obtained from the American Type Culture Collection (ATCC)) were cultured in Eagle's Minimum Essential Medium (EMEM) (ATCC® 30-2003TM) supplemented with 15% (volume/volume) fetal bovine serum (FCS), 100 U/mL penicillin, and 100 μg/mL streptomycin (Sigma-Aldrich). ..

    Article Title: Proteome profiling of large extracellular vesicles reveals distinct molecular signatures associated with breast cancer brain metastasis
    Article Snippet: The breast cancer cell lines MDA-MB-231, MDA-MB-361, and MCF7 were obtained from the American Type Culture Collection (ATCC, Manassas, VA), while the brain-seeking variant MDA-MB-231BR was kindly provided by Dr. Paul Lockman (West Virginia University, School of Pharmacy, Morgantown, WV). .. Cell culture media, including Dulbecco’s Modified Eagle’s Medium (DMEM), Eagle's Minimum Essential Medium (EMEM), Roswell Park Memorial Institute Medium (RPMI-1640), fetal bovine serum (FBS), penicillin-streptomycin, and phosphate-buffered saline (PBS) were purchased from ATCC. .. Trypsin-EDTA was obtained from Dow Corning, and culture flasks and pipettes were supplied by VWR.

    Article Title: Effects of sucralose on microbial and intestinal epithelial activity
    Article Snippet: Colorectal adenocarcinoma cell line, HT-29 (HTB-38TM, ATCC), was maintained at 37 ◦C in a humidified 5% CO2 incubator. .. Cells were initially cultured in Eagle's Minimum Essential Medium (EMEM) (302003, ATCC) with 10% FBS. .. Due to slow proliferation under EMEM, cells were switched to Dulbecco's Modified Eagle Medium (DMEM) (11995040, ThermoFisher) with 10% FBS.

    Modification:

    Article Title: Proteome profiling of large extracellular vesicles reveals distinct molecular signatures associated with breast cancer brain metastasis
    Article Snippet: The breast cancer cell lines MDA-MB-231, MDA-MB-361, and MCF7 were obtained from the American Type Culture Collection (ATCC, Manassas, VA), while the brain-seeking variant MDA-MB-231BR was kindly provided by Dr. Paul Lockman (West Virginia University, School of Pharmacy, Morgantown, WV). .. Cell culture media, including Dulbecco’s Modified Eagle’s Medium (DMEM), Eagle's Minimum Essential Medium (EMEM), Roswell Park Memorial Institute Medium (RPMI-1640), fetal bovine serum (FBS), penicillin-streptomycin, and phosphate-buffered saline (PBS) were purchased from ATCC. .. Trypsin-EDTA was obtained from Dow Corning, and culture flasks and pipettes were supplied by VWR.

    Article Title: Raman spectroscopic characterization of protein changes induced by 5-fluorouracil in colon cancer cells.
    Article Snippet: This paper aims to evaluate the current state of knowledge on understanding the impact of cytostatic on colon cancer progression.. We have compared the Raman profile of the normal colon fibroblast cell line (CCD-18Co) and two colon epithelial cancer cell lines (Caco-2 and LoVo).. Our results indicate that ratios 1656/1305, 1256/1305, and 1656/1256 are the most relative Raman biomarkers for discrimination between normal fibroblast and cancer epithelial colon cells.

    Saline:

    Article Title: Proteome profiling of large extracellular vesicles reveals distinct molecular signatures associated with breast cancer brain metastasis
    Article Snippet: The breast cancer cell lines MDA-MB-231, MDA-MB-361, and MCF7 were obtained from the American Type Culture Collection (ATCC, Manassas, VA), while the brain-seeking variant MDA-MB-231BR was kindly provided by Dr. Paul Lockman (West Virginia University, School of Pharmacy, Morgantown, WV). .. Cell culture media, including Dulbecco’s Modified Eagle’s Medium (DMEM), Eagle's Minimum Essential Medium (EMEM), Roswell Park Memorial Institute Medium (RPMI-1640), fetal bovine serum (FBS), penicillin-streptomycin, and phosphate-buffered saline (PBS) were purchased from ATCC. .. Trypsin-EDTA was obtained from Dow Corning, and culture flasks and pipettes were supplied by VWR.

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    LINC00887 is highly expressed and its impact on aerobic glycolysis in ccRCC, (A) Analysis of LINC00887 mRNA levels in ccRCC and adjacent normal tissues from TCGA-KIRC dataset. (B) qRT-PCR test of LINC00887 mRNA levels in HK-2, 786-O, Caki-1, and A498 cell lines. (C) KEGG pathway analysis of signaling pathways enriched by LINC00887 in ccRCC. (D) KEGG analysis showing enrichment of LINC00887 in the glycolysis pathway in ccRCC. (E) Pearson correlation analysis between LINC00887 and glycolytic marker genes. 786-O cells were transfected with either oe-NC or oe-LINC00887, respectively. (F) qRT-PCR was performed to verify transfection efficiency. (G) qRT-PCR measurement of glycolytic marker genes LDH-A, HK2, and GLUT1 mRNA levels in each group. (H) immunoblotting analysis of glycolytic marker LDH-A, HK2, and GLUT1 protein levels in each group. (I) Seahorse metabolic analyzer was used to measure ECAR in each group, calculating glycolytic value and glycolytic capacity. (J) Seahorse metabolic analyzer was used to measure OCR in each group, calculating ATP production, and maximal respiration. (K–M) Kits were used to measure glucose consumption (K) , lactate production (L) , and ATP yield (M) . *indicates p < 0.05. ccRCC, Clear cell renal cell carcinoma; qRT-PCR, Quantitative reverse transcription polymerase chain reaction; KEGG, Kyoto Encyclopedia of Genes and Genomes; LDH-A, Lactate dehydrogenase A; HK2, Hexokinase 2; GLUT1, Glucose transporter 1; ECAR, Extracellular acidification rate; OCR, Oxygen consumption rate; ATP, Adenosine triphosphate.

    Journal: Frontiers in Immunology

    Article Title: UPF1-dependent glycolysis driven by LINC00887 facilitates macrophage M2 polarization in clear cell renal cell carcinoma

    doi: 10.3389/fimmu.2026.1833383

    Figure Lengend Snippet: LINC00887 is highly expressed and its impact on aerobic glycolysis in ccRCC, (A) Analysis of LINC00887 mRNA levels in ccRCC and adjacent normal tissues from TCGA-KIRC dataset. (B) qRT-PCR test of LINC00887 mRNA levels in HK-2, 786-O, Caki-1, and A498 cell lines. (C) KEGG pathway analysis of signaling pathways enriched by LINC00887 in ccRCC. (D) KEGG analysis showing enrichment of LINC00887 in the glycolysis pathway in ccRCC. (E) Pearson correlation analysis between LINC00887 and glycolytic marker genes. 786-O cells were transfected with either oe-NC or oe-LINC00887, respectively. (F) qRT-PCR was performed to verify transfection efficiency. (G) qRT-PCR measurement of glycolytic marker genes LDH-A, HK2, and GLUT1 mRNA levels in each group. (H) immunoblotting analysis of glycolytic marker LDH-A, HK2, and GLUT1 protein levels in each group. (I) Seahorse metabolic analyzer was used to measure ECAR in each group, calculating glycolytic value and glycolytic capacity. (J) Seahorse metabolic analyzer was used to measure OCR in each group, calculating ATP production, and maximal respiration. (K–M) Kits were used to measure glucose consumption (K) , lactate production (L) , and ATP yield (M) . *indicates p < 0.05. ccRCC, Clear cell renal cell carcinoma; qRT-PCR, Quantitative reverse transcription polymerase chain reaction; KEGG, Kyoto Encyclopedia of Genes and Genomes; LDH-A, Lactate dehydrogenase A; HK2, Hexokinase 2; GLUT1, Glucose transporter 1; ECAR, Extracellular acidification rate; OCR, Oxygen consumption rate; ATP, Adenosine triphosphate.

    Article Snippet: Caki-1 cells were kept in 5A medium (30-2007, ATCC, USA), 786-O cells in DMEM-H (11965092, Gibco, USA), and A498 cells in EMEM (30-2003, ATCC, USA).

    Techniques: Analysis, Quantitative RT-PCR, Protein-Protein interactions, Marker, Transfection, Western Blot, Reverse Transcription, Polymerase Chain Reaction

    LINC00887-mediated UPF1 suppresses ccRCC malignant progression, (A) mRNA levels of UPF1 in ccRCC tumor tissues versus adjacent normal tissues as analyzed by TCGA-KIRC dataset. (B) qRT-PCR detection of UPF1 mRNA in HK-2, Caki-1, 786-O, and A498 cells. (C) Immunoblotting assessment of UPF1 protein in renal cell lines. (D) CCK-8 assay for cell viability in 786-O cells expressing sh-NC or sh-UPF1 and Caki-1 cells expressing oe-NC or oe-UPF1. (E) Colony formation assay determined cell proliferation in the transfected cells. (F) Scratch assay evaluated cell migration. (G) Transwell assessed cell invasion. (H) Flow cytometry analyzed apoptosis in different cell groups. (I) Flow cytometry examined cell cycle distribution in various cell groups. *, p < 0.05. ccRCC, Clear cell renal cell carcinoma; UPF1, Up-frameshift protein 1. qRT-PCR, Quantitative reverse transcription polymerase chain reaction; TCGA, The cancer genome atlas; KIRC, Kidney renal clear cell carcinoma.

    Journal: Frontiers in Immunology

    Article Title: UPF1-dependent glycolysis driven by LINC00887 facilitates macrophage M2 polarization in clear cell renal cell carcinoma

    doi: 10.3389/fimmu.2026.1833383

    Figure Lengend Snippet: LINC00887-mediated UPF1 suppresses ccRCC malignant progression, (A) mRNA levels of UPF1 in ccRCC tumor tissues versus adjacent normal tissues as analyzed by TCGA-KIRC dataset. (B) qRT-PCR detection of UPF1 mRNA in HK-2, Caki-1, 786-O, and A498 cells. (C) Immunoblotting assessment of UPF1 protein in renal cell lines. (D) CCK-8 assay for cell viability in 786-O cells expressing sh-NC or sh-UPF1 and Caki-1 cells expressing oe-NC or oe-UPF1. (E) Colony formation assay determined cell proliferation in the transfected cells. (F) Scratch assay evaluated cell migration. (G) Transwell assessed cell invasion. (H) Flow cytometry analyzed apoptosis in different cell groups. (I) Flow cytometry examined cell cycle distribution in various cell groups. *, p < 0.05. ccRCC, Clear cell renal cell carcinoma; UPF1, Up-frameshift protein 1. qRT-PCR, Quantitative reverse transcription polymerase chain reaction; TCGA, The cancer genome atlas; KIRC, Kidney renal clear cell carcinoma.

    Article Snippet: Caki-1 cells were kept in 5A medium (30-2007, ATCC, USA), 786-O cells in DMEM-H (11965092, Gibco, USA), and A498 cells in EMEM (30-2003, ATCC, USA).

    Techniques: Quantitative RT-PCR, Western Blot, CCK-8 Assay, Expressing, Colony Assay, Transfection, Scratch Assay, Migration, Flow Cytometry, Reverse Transcription, Polymerase Chain Reaction